Geraldine Seydoux

Academic Titles: 
Professor
410-614-4622

725 N. Wolfe Street

Baltimore, MD 21205

Publications

Research Interest: 
Molecular Genetics of Embryonic Polarity and Germ Cell Development

Our lab studies the earliest stages of embryogenesis to understand how single-celled eggs develop into complex multicellular embryos. We focus on the choice between soma and germline, one of the first developmental decisions faced by embryos. Our goal is to identify and characterize the molecular mechanisms that activate embryonic development, polarize embryos, and distinguish between somatic and germline cells. We use genetic, molecular and biochemical techniques and use the Caenorhabditis elegans as a model system.

 


P granules assemble in the posterior cytoplasm of a wild-type zygote, but not in a zygote lacking MEG-3 and MEG-4, two intrinsically-disorderedproteins required for P granule assembly. Jenn Wang and Geraldine Seydoux

 

Lattice light sheet microscopy reveals the "skeleton" of RNA granules: each granule is held together by a dynamic, discontinuous ribbon that threads around and through the granule.

 

Biosketch: 
Lab Members:
Selected Publications:
Lee, CY, Putnam, A, Lu T., Seydoux, G 2019. Recruitment of mRNAs to P granules by gelation with intrinsically-disordered proteins. http://biorxiv.org/cgi/content/short/732057v1. [ pdf ]
Ouyang, JPT, Folkmann, A, Bernard, L, Lee, CY, Seroussi, U, Charlesworth, AG et al. P Granules Protect RNA Interference Genes from Silencing by piRNAs. Dev. Cell. 2019; :. doi: 10.1016/j.devcel.2019.07.026. [ pdf ]
Putnam, A, Cassani, M, Smith, J, Seydoux, G. A gel phase promotes condensation of liquid P granules in Caenorhabditis elegans embryos. Nat. Struct. Mol. Biol. 2019;26 (3):220-226. doi: 10.1038/s41594-019-0193-2. [ pdf ]
Folkmann, AW, Seydoux, G. Spatial regulation of the polarity kinase PAR-1 by parallel inhibitory mechanisms. Development. 2019;146 (6):. doi: 10.1242/dev.171116.  [ pdf ]
Lee, CY, Seydoux, G. Dynamics of mRNA entry into stress granules. Nat. Cell Biol. 2019;21 (2):116-117. doi: 10.1038/s41556-019-0278-5. [ pdf ]
Paix A, Folkmann A, Goldman DH, Kulaga H, Grzelak MJ, Rasoloson D, Paidemarry S, Green R, Reed RR, Seydoux G. (2017) Precision genome editing using synthesis-dependent repair of Cas9-induced DNA breaks. Proc Natl Acad Sci U S A. (2017) Dec 12;114(50):E10745-E10754. [ link ]
Mok CA, Au V, Thompson OA, Edgley ML, Gevirtzman L, Yochem J, Lowry J, Memar N, Wallenfang MR, Rasoloson D, Bowerman B, Schnabel R, Seydoux G, Moerman DG, Waterston RH. (2017) MIP-MAP: High-Throughput Mapping of Caenorhabditis elegans Temperature-Sensitive Mutants via Molecular Inversion Probes. Genetics Oct;207(2):447-463.  [ link ]
Smith J, Calidas D, Schmidt H, Lu T, Rasoloson D, Seydoux G. (2016) Spatial patterning of P granules by RNA-induced phase separation of the intrinsically-disordered protein MEG-3. eLife 2016;10.7554/eLife.21337. [ link ]
Paix A, Folkmann A, Rasoloson D, Seydoux G. (2015) High Efficiency, Homology-Directed Genome Editing in Caenorhabditis elegans Using CRISPR-Cas9 Ribonucleoprotein Complexes. Genetics 201(1):47-54. [ pdf ]
Wang JT, Smith J, Chen BC, Schmidt H, Rasoloson D, Paix A, Lambrus BG, Calidas D, Betzig E, Seydoux G. (2014) Regulation of RNA granule dynamics by phosphorylation of serine-rich, intrinsically disordered proteins in C. elegans. Elife Dec 23;3:e04591. [ link ]
Seydoux G. (2013) 'Goldilocks' suppressor screen identifies web of polarity regulators. Nature Cell Biology 15(1):9-10. [ link ] [ pdf ]
Griffin EE, Odde DJ, Seydoux G. (2011) Regulation of the MEX-5 gradient by a spatially segregated kinase/phosphatase cycle. Cell 146(6):955-68. [ link ]
Gallo CM, Wang JT, Motegi F, Seydoux G. (2010) Cytoplasmic partitioning of P granule components is not required to specify the germline in C. elegans. Science 330(6011):1685-9. [ link ]