Geraldine Seydoux

Academic Titles: 
Professor
410-614-4622

725 N. Wolfe Street

Baltimore, MD 21205

Research Interest: 
Molecular Genetics of Embryonic Polarity and Germ Cell Development

Our lab studies the earliest stages of embryogenesis to understand how single-celled eggs develop into complex multicellular embryos. We focus on the choice between soma and germline, one of the first developmental decisions faced by embryos. Our goal is to identify and characterize the molecular mechanisms that activate embryonic development, polarize embryos, and distinguish between somatic and germline cells. We use genetic, molecular and biochemical techniques and use the Caenorhabditis elegans as a model system.

 

P granules assemble in the posterior cytoplasm of a wild-type zygote, but not in a zygote lacking MEG-3 and MEG-4, two intrinsically-disorderedproteins required for P granule assembly. Jenn Wang and Geraldine Seydoux

 

Lattice light sheet microscopy reveals the "skeleton" of RNA granules: each granule is held together by a dynamic, discontinuous ribbon that threads around and through the granule.

 

Biosketch: 
Lab Members:
Name Classificationsort descending Email
Alexandre Paix Research Associate
Alex Paix Postdoctoral Fellow apaix1@jhmi.edu
Andrew Folkmann Postdoctoral Fellow afolkma1@jhu.edu
Chih-Yung (Sean) Lee Postdoctoral Fellow clee135@jhmi.edu
Andrea Putnam Postdoctoral Fellow
Dominique Rasoloson Research Specialist drasolo1@jhmi.edu
Madeline Cassani Graduate Student
John Paul Ouyang Graduate Student
Tu Lu Graduate Student tlu16@live.johnshopkins.edu
Helen Schmidt Graduate Student hschmi11@jhmi.edu
Jarrett Smith Graduate Student jarrett.smith27@gmail.com
Selected Publications:
Smith J, Calidas D, Schmidt H, Lu T, Rasoloson D, Seydoux G. (2016) Spatial patterning of P granules by RNA-induced phase separation of the intrinsically-disordered protein MEG-3. eLife 2016;10.7554/eLife.21337. [ link ]
Paix A, Folkmann A, Rasoloson D, Seydoux G. (2015) High Efficiency, Homology-Directed Genome Editing in Caenorhabditis elegans Using CRISPR-Cas9 Ribonucleoprotein Complexes. Genetics 201(1):47-54. [ pdf ]
Wang JT, Smith J, Chen BC, Schmidt H, Rasoloson D, Paix A, Lambrus BG, Calidas D, Betzig E, Seydoux G. (2014) Regulation of RNA granule dynamics by phosphorylation of serine-rich, intrinsically disordered proteins in C. elegans. Elife Dec 23;3:e04591. [ link ]
Seydoux G. (2013) 'Goldilocks' suppressor screen identifies web of polarity regulators. Nature Cell Biology 15(1):9-10. [ link ] [ pdf ]
Griffin EE, Odde DJ, Seydoux G. (2011) Regulation of the MEX-5 gradient by a spatially segregated kinase/phosphatase cycle. Cell 146(6):955-68. [ link ]
Gallo CM, Wang JT, Motegi F, Seydoux G. (2010) Cytoplasmic partitioning of P granule components is not required to specify the germline in C. elegans. Science 330(6011):1685-9. [ link ]