A natural single-guide RNA repurposes Cas9 to autoregulate CRISPR-Cas expression

Work by RE Workman and T Pammi from the Modell lab:

CRISPR-Cas systems provide their prokaryotic hosts with acquired immunity against viruses...

Multiplexed monitoring to track single cell signaling dynamics

We highlight the research of the Regot Lab:


A fundamental property of living cells is their extraordinary ability to sense and...

Scalable and versatile genome editing using linear DNAs with microhomology to Cas9 Sites in Caenorhabditis elegans

Work by Alex Paix from the Seydoux Lab:

"Homology-directed repair (HDR) of double-strand DNA breaks is a promising method for genome...

Recent Publications

Marie J. StoltzfusRachael E. WorkmanNicholas C. KeithJoshua W. Modell

Mandlbauer A, Sun Q, Popitsch N, Schwickert T, Spanova M, Wang J, Ameres SL, Busslinger M, Cochella L. Mime-seq 2.0: a method to sequence microRNAs from specific mouse cell types. EMBO J. 2024 Apr 30. doi: 10.1038/s44318-024-00102-8. PMID: 38689024

Workman RE, Stoltzfus MJ, Keith NC, Euler CW, Bondy-Denomy J, Modell JW. Anti-CRISPR proteins trigger a burst of CRISPR-Cas9 expression that enhances phage defense. Cell Rep. 2024 Mar 26;43(3):113849. doi: 10.1016/j.celrep.2024.113849. Epub 2024 Feb 29. PMID: 38427560; PMCID: PMC11078563. [ link ]

Zhao S, Cordes J, Caban KM, Götz MJ, Mackens-Kiani T, Veltri AJ, Sinha NK, Weickert P, Kaya S, Hewitt G, Nedialkova DD, Fröhlich T, Beckmann R, Buskirk AR, Green R, Stingele J. RNF14-dependent atypical ubiquitylation promotes translation-coupled resolution of RNA-protein crosslinks. Mol Cell. 2023 Dec 7;83(23):4290-4303.e9. doi: 10.1016/j.molcel.2023.10.012. Epub 2023 Nov 10. PMID: 37951216. Link

Coller, J., Ignatova, Z. tRNA therapeutics for genetic diseases. Nat Rev Drug Discov (2023).

Black JJ, Green R. Saving ribosomal proteins for later. Nat Cell Biol. 2023 Nov;25(11):1568-1569. doi: 10.1038/s41556-023-01251-4. PMID: 37845326. Link

Saba JA, Huang Z, Schole KL, Ye X, Bhatt SD, Li Y, Timp W, Cheng J, Green R. LARP1 senses free ribosomes to coordinate supply and demand of ribosomal proteins. bioRxiv [Preprint]. 2023 Nov 2:2023.11.01.565189. doi: 10.1101/2023.11.01.565189. PMID: 37961604; PMCID: PMC10635049. Link

Upcoming Events

August 2024

Thu, Aug 1

11:00 am – 12:00 pm
MBG Seminar, Marvin Tanenbaum, Ph.D., Hubrecht Institute

September 2024

Thu, Sep 5

9:30 am – 12:30 pm
Reserved for MBG Seminars
11:00 am – 12:30 pm
MBG Colloquium: Seydoux Lab

Thu, Sep 12

9:30 am – 12:30 pm
Reserved for MBG Seminars
11:00 am – 12:00 pm
MBG Seminar: Toshifumi Inada